Molecular thermodynamics for protein precipitation with a polyelectrolyte
Article Abstract:
A molecular-thermodynamic model for phase equilibria was developed in an effort to assess the protein precipitation of charged globular proteins in an aqueous environment. The proposed framework makes use of charged-hard sphere chain to denote linear polyelectrolyte and a spherical macroion to represent globular proteins. Results revealed that precipitation is enhanced through the addition of polyelectrolyte into a protein solution and further addition leads to the redesolution of these compounds.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 1999
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Lysozyme net charge and ion binding in concentrated aqueous electrolyte solutions
Article Abstract:
A study was conducted to analyze lysozyme net charge and ion bonding in electrolyte solutions of potassium chloride over the pH range 2.5-11.5 and ionic strengths to 2.0 M. A serial addition of aliquots of an alkaline titrant solution was utilized to carry out calibration titrations. Results indicated the dependency of ion-protein interactions on pH and ionic strength that is correlated with electric-double-layer theory.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 1999
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Measurement of lysozyme-lysozyme interactions with quantitative affinity chromatography
Article Abstract:
The quantitative affinity chromatography is used to measure the lysozyme-lysozyme interactions at a variety of solution conditions. The interactions between lysozyme molecules are described by a potential of mean force that contains hard-sphere, electrostatic, and square-well contributions.
Publication Name: Journal of Physical Chemistry B
Subject: Chemicals, plastics and rubber industries
ISSN: 1520-6106
Year: 2004
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