Quantification of surface-bound proteins by fluorometric assay: Comparison with Quartz crystal microbalance and amide black assay

Article Abstract:

The use of Nano Orange, a fluorometric assay is used to quantitatively assess the adsorption of bovine fibrinogen and albumin onto model hydrophilic (OH terminated) and hydrophobic (CH3 terminated) surfaces. The study demonstrates the potential of a fluorometric assay to be used as an external calibration for other techniques following protein adsorption processes or as a supplemental method to study protein adsorption.

Fibrinogen, Fluorimetry, Albumin, Structure

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Engineering a beta-sheet protein toward the folding speed limit

Article Abstract:

A study is conducted to demonstrate how the hPin1 WW domain, a triple-stranded beta-sheet protein with a sharp thermodynamic melting transition can be engineered toward the folding 'speed limit' without a significant loss in thermal denaturation cooperatively. Triple-stranded beta-sheet protein hPin1 can be reengineered in loop 1 to increase the folding rate by over a factor of 10 to greater than 0.1 (mu)S(super -1).

Protein folding

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Mechanisms of silk fibroin sol-gel transitions

Article Abstract:

A study on the silk fibroin sol-gel transitions is conducted by monitoring the process under various physicochemical conditions with optical spectroscopy at 550 nm. No detectable changes in the protein secondary structure are found up to about 15% gelation, indicating that these early stages of gelation are not accompanied by the formation of [beta]-sheets.

Analysis, Spectrum analysis, Spectroscopy, Gelation

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