Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation

Article Abstract:

It is possible to prevent chromatin nucleating microtubules by inhibiting RCC1, the guanine-nucleotide-exchange factor for the GTPase protein Ran, from producing Ran-GTP by RanT24N. This can also be achieved by boosting the rate of reconversion of Ran-GTP generated by RCC1 activity back to the GDP-bound state by using excess ranBP1 and ranGAP. The activity of chromosome-associated RCC1 protein is needed for spindle formation. This research has provided a molecular basis for the impact of chromosomes on local microtubule growth seen in dividing plant cells and meiotic animal cells.

Chromatin, Microtubules

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RanGTP mediates nuclear pore complex assembly

Article Abstract:

RanGTP triggering distinct steps of nuclear pore complexes (NPC) assembly is demonstrated and a mechanism for the spatial restriction of NPC assembly to the surface of chromatin is suggested. NPC, assemble into the reforming nuclear envelope in a sequential process involving association of a subset of NPC proteins, nucleoporins, with chromatin followed by the formation of a closed nuclear envelope fenestrated by NPCs (super 2-7).

Cell nuclei, Cell nucleus

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Structural basis for nuclear import complex dissociation by RanGTP

Article Abstract:

The structure of full-length yeast importin-beta complexed with RanGTP, which provides a basis for understanding the crucial cargo release step of nuclear import, is investigated. An allosteric mechanism for nuclear import complex disassembly by RanGTP is suggested.

Dissociation, Dissociation reactions, Cytoplasm, GTP, Guanosine triphosphate

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