Structural basis for initiation of transcription from an RNA polymerase-promoter complex

Article Abstract:

Bacteriophage T7 (T7RNAP) is homologous to the Pol I group of DNA polymerases. The crystal structure of T7RNAP complexed with a 17-base pair promoters reveals four base pairs near to the catalytic active site that melt to form a transcription bubble. The T7 promoter sequence is recognized by interactions between antiparallel beta-loop and bases. The specificity of T7RNAP for ribonucleotides is explained.

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Activity of RNA polymerase I transcription factor UBF blocked by Rb gene product

Article Abstract:

The suppression of cell growth by the retinoblastoma susceptibility gene (Rb) occurs by a new process, which involves the direct repression of the rRNA gene transcription. The Rb functions as a negative growth regulator and tumor suppressor in the human body. The new process by which suppression occurs is detected by immunoprecipitation and affinity chromalography.

Retinoblastoma

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Protein-primed RNA synthesis by purified poliovirus RNA polymerase

Article Abstract:

The 5' end of the plus-stranded poliovirus genomic RNA is covalently bonded to the small protein VPg, which has only 22 amino acids. Genomic RNA has an identical nucleotide sequence to poliovirus messenger RNA, which is not capped at its 5' end. New research investigates the function of VPg, which is shown can be uridylylated by the poliovirus RNA polymerase 3Dpol.

Messenger RNA, Poliovirus, Polioviruses

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