A bead-based method for multiplexed identification and quantitation of DNA sequences using flow cytometry

Article Abstract:

Results demonstrate that a direct hybrid capture assay in a multiplexed, bead-based format incorporating nucleic acid hybridization on the surface identifies specific sequences in a mixture of DNA sequences. Data reveal that the bead method exhibits superior sequence discrimination capabilities and detects quantitatively the unknown DNA sequences.

Testing, DNA, Nucleotide sequence, Base sequence

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Stimulating the in situ activity of Geobacter species to remove uranium from the groundwater of a uranium-contminated aquifer

Article Abstract:

Research reveals that in situ bioremediation of uranium-contaminated groundwater can be accomplished by maintaining long-term activity of Geobacter species. Data show that addition of acetate to the groundwater stimulates the growth of Geobacter species, which coupled with their long-term growth remove uranium from the groundwater in situ.

Primary nonferrous metals, not elsewhere classified, Primary Smelting and Refining of Nonferrous Metal (except Copper and Aluminum), All Other Basic Inorganic Chemical Manufacturing, Uranium Metal, Industrial inorganic chemicals, not elsewhere classified, Reprocessed Activated Uranium, Bacterial growth, Bioremediation, Water, Underground, Groundwater, Contamination, Uranium, Fuel reprocessing (Nuclear reactors)

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Quantitative assessment of picoeukaryotes in the natural environment by using taxon-specific oligonucleotide probes in association with tyramide signal amplification-fluorescence in situ hybridization and flow cytometry

Article Abstract:

Research has been conducted on picoeukaryotes. The authors describe the protocol developed for the quantification of these picoeukaryotes in the natural environment via the use of flow cytometry and tyramide signal amplification-fluorescence in situ hybridization.

France, Biological Product (except Diagnostic) Manufacturing, Nucleic Acid Derivatives, Genetic aspects, Eukaryotes, Microbiology, Nucleotides, Microbial populations, Oligomers, Microbial ecology

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