A coiled-coil from the RNA polymerase beta' subunit allosterically induces selective nontemplate strand binding by sigma(sup)70

Article Abstract:

Results indicate that the beta' coiled-coil and sigma(sup)70 region interact and promote an allosteric transition that enables sigma(sup)70 to selectively recognize the nontemplate strand. Data reveal that a 48 amino acid coiled-coil of the beta' subunit induces sigma(sup)70 to perform the task as efficiently as core RNA polymerase.

Enzymes, Genetic regulation, RNA polymerases, Enzyme structure-activity relationships, Allosteric enzymes

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Luminescence resonance energy transfer-based high-throughput screening assay for inhibitors of essential protein-protein interactions in bacterial RNA polymerase

Article Abstract:

This article describes a homogeneous assay for sigma factor binding to RNA polymerase based on luminescence resonance energy transfer. Data indicate that the assay can be performed in a multiwell plate and read by amultiplate reader enabling it to be a robust high-throughput screening assay for protein-proein interaction.

Methods, Measurement, Proteins, Luminescence, Resonance, Resonance (Physics), Assaying

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OMP peptide signals initiate the envelope-stress response by activating DegS protease via relief of inhibition mediated by its PDZ domain

Article Abstract:

Results show that outer-membrane porins-like C-terminal sequences bind to DegS protease PDZ domain and activate DegS cleavage of transmembrane protein RseA to induce sigma(sup)E -dependent transcription. Data indicate that DegS senses envelope stress by binding to unassembled outer membrane porins.

Membrane proteins, Cellular signal transduction, Enzyme activation, Porins

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