A fermentor system for regulating oxygen at low concentrations in cultures of Saccharomyces cerevisiae

Article Abstract:

A system comprised of a computer-controlled gas flow train that adjusts oxygen concentration in the gas flow to match cellular demand. This is achieved by mixing two gases and adjusting their proportions to maintain a desired oxygen concentration. The system was designed to circumvent the problem of increasing oxygen consumption as cell mass increases in the growth of yeast cells to high densities at low yet constant oxygen concentrations. Yeast cell yields at intermediate to low oxygen concentrations were modeled, finding that cellular respiration declines with oxygen concentration.

Oxygen consumption, Oxygen consumption (Metabolism)

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Thiram and dimethyldithiocarbamic acid intervconversion in Saccharomyces cerevisiae: a possible metabolic pathway under the control of the glutathione redox cycle

Article Abstract:

A study on thiram and dimethyldithiocarbamic acid interconversion in Saccharomyces cerevisiae was conducted to determine the possible metabolic pathway under the control of gluthathione redox cycle. The research entailed thetreatment of the Saccharomyces cerevisiae cells with sublethal concentrations of dimethyldithiocarbamic acid. Results reveal that the redox couple thiram acts a link while the intracellular gluthathione acts as a pathway that provides reducing equivalents for thiram bioreduction.

Metabolic regulation

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Inactivation of MXR1 abolishes formation of dimethyl sulfide from dimethyl sulfoxide in Saccharomyces cerevisiae

Article Abstract:

Research was conducted to examine the hypothesis that MXR1 encodes an enzyme responsible for both the MetSO reduction and reduction of dimethyl sulfoxide to dimethyl sulfide during fermentation of brewer's wort. Results support the hypothesis that reduction of peptide methionine sulfoxides is the physiological function of the polypeptide encoded by open reading frame YER042W. The name MXR1 for peptide methionine sulfoxide reductase 1 is suggested for the gene.

Beer, Dimethyl sulfoxide, Saccharomyces cerevisiae

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