A novel means to develop strain-specific DNA probes for detecting bacteria in the environment

Article Abstract:

The means to develop strain-specific DNA probes for detecting bacteria in the environment was examined using the polymerase chain reaction (PCR) amplification of target sequences. The method was used without imposing the need to determine the nucleic acid sequence of the probe to produce the PCR primers. Results reveal that the probes can be developed and utilized without prior knowledge of the host strain and that DNA probe sensitivity and selectivity observed conform with those in other PCR-based methods.

Usage, Polymerase chain reaction, DNA probes

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Pristine environments harbor a new group of oligotrophic 2,4-dichlorophenoxyacetic acid-degrading bacteria

Article Abstract:

2,4-Dichlorophenoxyacetic acid (2,4-D)-degrading bacteria were isolated from pristine environments which had no history of 2,4-D exposure. Findings showed that 2,4-D-degrading microorganisms are widespread in pristine environments but are very difficult to culture. Also, most of the 2,4-D degraders in pristine soils are slow-growing bacteria and are sensitive to high concentrations of organic nutrients.

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System for determining the relative fitness of multiple bacterial populations without using selective markets

Article Abstract:

A study developed and evaluated a device for simultaneously measuring the relative fitness of multiple bacterial populations. The new device eliminates the need to construct strains with selectivity neutral markers so that strains could be readily distinguished, and it provides a means to perform multispecies competition experiments.

Science & research

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