A primer on vesicle budding

Article Abstract:

A model for transport vesicle formation is reported. This model revolves around the priming complex, which is formed by a primer protein, a small GTPase, and one or more subunits of the vesicular coat, including a protein that activates GTPase. It is theorized that vesicle formation occurs in four stages: GTPase activation and membrane binding, priming complex formation, coat oligomerization and loss of the GTPase, and vesicle budding.

Analysis, Cell research, Cytological research, Cell organelles, Organelles, Enzyme activation

---

Mammalian Pins is a conformational switch that links NuMA to heterotrimeric G proteins

Article Abstract:

A mammalian Pins, called LGN, binds G alpha i and interacts through an N-terminal domain with the microtubule binding protein NuMA. A FRET biosensor is used in a study to demonstrate that LGN acts as a conformational switch in its closed state, the N and C termini interact, but NuMA or G alpha i can disrupt this association, allowing LGN to interact simultaneously with both proteins, resulting in their cortical localization.

Genetic aspects, Biosensors, Protein binding, Mammals

---

Closing the GAP between polarity and vesicle transport

Article Abstract:

Polarity proteins may be required for the establishment of tight junctions and transmembrane proteins of the tight junctions must be delivered to the plasma membrane in vesicles. Evidence is provided for a new pathway that links polarity proteins and vesicle transport to the maintenance of tight junctions, through the control of Cdc42 by Rich1, a GTPase-activating protein.

Cell membranes, Genetic research, Guanosine triphosphatase, GTPases

Similar abstracts:

• Abstracts: Chaperone-mediated folding and maturation of the Penicillin acylase precursor in the cytoplasm of Escherichia coli
• Abstracts: Architecture of the Hin synaptic complex during recombination: The recombinase subunits translocate with the DNA strands
• Abstracts: Endocytosis conducts the cell signaling orchestra. Heterotrimeric G protein signaling: Getting inside the cell
• Abstracts: Anaerobic sulfide oxidation with nitrate by a freshwater Beggiatoa enrichment culture
• Abstracts: Toxoplasma invasion of mammalian cells is powered by the actin cytoskeleton of the parasite. Toxoplasma: Guess who's coming to dinner

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.