A revolutionary approach to biomarker discovery

Article Abstract:

A research work to develop a panel of candidate peptide biomarkers along with measurement reagents that are independent of the analytical technology used in the clinical lab to determine cancer biomarkers is presented. As new classes of clinical mass spectrometry (MS) technologies emerge and methodologies such as immune-MS become evaluated and developed for routine clinical use, researchers are more optimistic about the emergence and newfound appreciation of the circulatory peptidome that would have a positive impact on patient care.

Product development, Immunoassay, Biological markers

---

Serum proteomics scrutinized

Article Abstract:

The surface-enhanced laser desorption/ionization-time-of-flight (SELDI-TOF) mass spectrometry would prove to be an important method in diagnosing cancer at an early stage, however, critics maintained that the process lacked sensitivity needed to detect low-abundance proteins. Three different experimental methods were combined, and compared to develop a nonredundant list of proteins in human plasma and serum, finding that no single platform provided complete coverage of the proteome.

Research and Development in the Physical, Engineering, and Life Sciences, Research and Testing Services, Cancer & Cell R&D, Cancer research, Cancer, Oncology, Experimental

---

Time-lapse proteomics

Article Abstract:

A method, based on stable isotope-labeled amino acids in cell culture, is developed that has combined large-scale quantitative proteomics with a technique for time-labeling cell cultures. The dynamics of growth factor signaling is characterized by stimulating isotope-tagged batches of cells with epidermal growth factor for different time intervals and the combined lysate is analyzed by using mass spectrometry.

Cell culture, Amino acid metabolism

Similar abstracts:

• Abstracts: Evaluation of a macrofoam swab protocol for the recovery of Bacillus anthracis spores from a steel surface. Inactivation of spores of Bacillus anthracis sterne, Bacillus cereus and Bacillus thuringiensis subsp. israelensis by chlorination
• Abstracts: Venom evolution widespread in fishes: A phylogenetic road map for the bioprospecting of piscine venoms . Why cavefish are blind
• Abstracts: Approaches to defining the ancestral eukaryotic protein complexome. Proteasome and transcription: A destroyer goes into construction
• Abstracts: A primer on vesicle budding. Mammalian Pins is a conformational switch that links NuMA to heterotrimeric G proteins
• Abstracts: A newspaper hires an IRB. Milk: It's electric. A waiting trial

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.