A simple method for quantification of uncultured microorganisms in the environment based on in vitro transcription of 16S rRNA

Article Abstract:

A novel method for quantitative hybridization was developed for the quantification of uncultured microorganisms based on the generation of nucleic acid standards. The method involves the quantitative estimate of a species added at different concentrations to an artificially assembled community based on ribosomal ribonucleic acids (rRNA) transcribed from cloned templates. Furthermore, the in vitro-transcribed rRNA can be utilized as a standard in quantitative probing of a microbial community's nucleic acids.

Methods, Analysis, Usage, Nucleic acid probes, Microbiology, Ribosomal RNA, Microbial populations

---

Bias in template-to-product ratios in multitemplate PCR

Article Abstract:

The potential causes and the extent of bias in polymerase chain reaction amplification of 16S ribosomal DNAs were investigated. Quantification and comparison of template and product ratios showed that there was considerable and reproducible overamplification of specific templates. Results indicated that bias may be reduced considerably by using high template concentrations, by performing fewer cycles and by mixing replicate reaction preparations.

Research, Polymerase chain reaction, Biochemical templates

---

Two new Mycobacterium strains and their role in toluene degradation in a contaminated stream

Article Abstract:

Two toluene-degrading Mycobacteria strains are isolated from a toluene-contaminated freshwater stream. The two strains have different rates of toluene degradation. Morphological, biochemical and genetic analysis of the isolated strains confirmed their relationship to the genus Mycobacteria. These fast-growing strains are closely related to Mycobacterium aurum and M. komossense based on the analysis of their 16S ribosomal RNA sequences.

Environmental aspects, Mycobacteria, Mycobacterium, Toluene, Aromatic compounds

Similar abstracts:

• Abstracts: A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization
• Abstracts: A molecular marker for the identification of the zoonotic reservoirs of Lyme borreliosis by analysis of the blood meal in its European vector Ixodes ricinus
• Abstracts: Molecular biological evidence for the occurrence of uncultured members of the actinomycete line of descent in different environments and geographical locations
• Abstracts: Biodegradation of free phytol by bacterial communities isolated from marine sediments under aerobic and denitrifying conditions
• Abstracts: Degradation of phthalate and di-(2-ethylhexyl)phthalate by indigenous and inoculated microorganisms in sludge-amended soil

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.