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A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization

Article Abstract:

A combination of Pro-Cipitate precipitation and polyethylene glycol (PEG) concentration techniques efficiently purifies enteric viruses in oyster extracts for identification by reverse transcriptase-PCR amplification and oligoprobe. The acid-alcohol polymer, Pro-Cipitate, selectively precipitates proteins from nucleic acids, glycoproteins and oligosaccharides. Seeded viruses in the oyster extracts are purified by fluorocarbon extraction and concentrated using PEG. The method is quick and sensitive and efficiently detects Norwalk virus even at low concentrations.

Author: Jaykus, Lee-Ann, Sobsey, Mark D., De Leon, Ricardo
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
Identification and classification, Polymerase chain reaction, Nucleic acid probes, Contamination, Oysters

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Detection of low levels of enteric viruses in metropolitan and airplane sewage

Article Abstract:

A polymerase chain reaction-based detection method was employed in the determination of enteric viruses in sewage. Application of this method to sewage coming from commercial airlines showed that sewage from international flights have enteroviruses which are infective based on cell culture studies. The results of this study suggest that international travel may contribute to the dissemination of enteric pathogens.

Author: Sobsey, Mark D., Baric, Ralph S., Shieh, Y.S. Carol
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
Disease transmission

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Detection of human enteric viruses in oysters by in vivo and in vitro amplification of nucleic acids

Article Abstract:

Two molecular techniques, nucleic acid amplification and oligonucleotide probing, were evaluated for their effectiveness in detecting human enteric viruses in oysters. A comparison of the number of positive samples detected by both methods revealed nucleic acid amplification as the more effective method for detecting noncytophatic human enteric viruses in oysters, with an efficiency of 50%.

Author: Jaykus, Lee-Ann, Sobsey, Mark D., Chung, Hyenmi
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
Analysis, Molecular biology, Enteroviruses

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Subjects list: Methods, Viruses
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