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A simple methodological approach for counting and identifying culturable viruses adsorbed to cellulose nitrate membrane filters

Article Abstract:

A technique for counting viruses in low-titer viral suspensions is reported. It uses Buffalo green monkey cells growing on a cellulose nitrate membrane to which the viruses are attached.

Author: Papageorgiou, Georgios T., Moce-Llivina, Laura, Christodoulou, Christina G., Lucena, Francisco, Akkelidou, Dina, Ioannou, Eleni, Jofre, Juan
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000

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New method for evaluation of virucidal activity of antiseptics and disinfectants

Article Abstract:

Researchers found the VIRADEN method to be as accurate as other methods for measuring virus activity in the presence of disinfectants and antiseptics. The VIRADEN method counts viruses adsorbed to cellulose nitrate filters. It was used to assess poliovirus activity in the presence of iodine, chlorine, glutaraldehyde, and chlorhexidine digluconate.

Author: Papageorgiou, Georgios T., Moce-Llivina, Laura, Jofre, Juan
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
Antiseptics, Disinfection and disinfectants, Disinfectants, Disinfection

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Survival of bacterial indicator species and bacteriophages after thermal treatment of sludge and sewage

Article Abstract:

Results reveal that bacteriophages are markedly more resistant to inactivation by heat treatment than bacterial indicator species as shown in sludge and sewage sources heated to 80 and 60 C, respectively. Further, somatic coliphages and phages are more resistant than F-specific RNA phages. Both sludge and sewage show similar inactivation patterns.

Author: Moce-Llivina, Laura, Lucena, Francisco, Jofre, Juan, Muniesa, Maite, Pimenta-Vale, Hugo
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
Spain, Sewage, Bacteriophages, Bacteria, Pathogenic, Pathogenic bacteria, Thermal properties, Sludge

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Subjects list: Measurement, Viruses, Evaluation
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