A soil and rhizosphere microorganism isolation and enumeration medium that inhibits Bacillus mycoides

Article Abstract:

The rhizosphere isolation medium (RIM) inhibits the growth of Bacillus mycoides which grows on the agar media and makes the study of other microorganisms difficult. The RIM consists of glucose and 15 of the 20 common amino acids and studies show that the 5 absent amino acids do not affect the growth of any microorganism other than B. mycoides because no bacteria group was found to grow only on agar and not on RIM.

Plant growing media

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A method for detection of pseudobactin, the siderophore produced by a plant-growth-promoting Pseudomonas strain, in the barley rhizosphere

Article Abstract:

The direct measurement of a pseudobactin siderophore in soil and rhizosphere samples by a monoclonal antibody and an enzyme-linked immunosorbent assay (ELISA) is discussed. Pseudomonas strains were subjected to plant innoculation, colonization assay and siderophore extraction. Rhizosphere samples underwent ELISA. The applicability of the combination of both techniques was demonstrated.

Pseudomonas, Rhizosphere

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Interpreting ecological diversity indices applied to terminal restriction fragment length polymorphism data: Insights from simulated microbial communities

Article Abstract:

Simulations are performed to determine whether diversity indices calculated from terminal restriction fragment length polymorphism (T-RFLP) profiles reflect the true diversity of the underlying communities despite potential analytical artifacts. It is concluded that T-RFLP based estimates of diversity provide inaccurate insights into the actual diversity of bacterial communities.

Science & research, Polymerase chain reaction, Genetic polymorphisms, Microbial ecology

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