ACF, an ISWI-containing and ATP-utilizing chromatin assembly and remodeling factor

Article Abstract:

The mechanisms that mediate the adenosine triphosphate (ATP)-dependence of chromatin assembly and remodeling was analyzed in Drosophila embryos during the initial fractionation of chromatin assembly. Analysis of chromatin assembly in Drosophila embryos indicated the presence of ATP-dependent nucleosome spacing modulation which was induced by the ATP-utilizing chromatin (ACF). Furthermore, ACF mediated the remodeling of chromatin by catalyzing nucleosome deposition and rearrangement.

Chromatin, Adenosine triphosphate, ATP

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Cdc53 is a scaffold protein for multiple Cdc34/Skp1/F-box protein complexes that regulate cell division and methionine biosynthesis in yeast

Article Abstract:

The E2 ubiquitin conjugation enzyme Cdc34 acts in conjunction with an E3 ubiquitin ligase complex composed of Skp1, Cdc53 and F-box protein, Cdc4 (the SCF super Cdc4 complex) to catalyze ubiquitination of the cyclin-dependent kinase (Cdk) inhibitor Sic1 in budding yeast. Cdc53 is a scaffold protein for various Cdc34/Skp1/F-box protein complexes that control yeast cell division and methionine biosynthesis. F-box proteins have great functional specificity in vivo.

Genetic aspects, Biosynthesis, Yeast, Yeast (Food product), Ubiquitin, Glucose, Methionine

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Cdc53 targets phosphorylated G1 cyclins for degradation by the ubiquitin proteolytic pathway

Article Abstract:

Proteins that interact with the G1 cyclin Cln2 that helps activate cell division in budding yeast are studied. Immunoaffinity column chromatography is used to identify the proteins, with a CLN2 allele being tagged with a triple hemagluttinin epitope to allow specific detection of Cln2-associated proteins. Cdc53 is isolated as a Cln2-associated protein and shown to be required for Cln2 instability and ubiquitination in vivo.

Cellular signal transduction

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