Accumulation of copper and other metals by copper-resistant plant-pathogenic and saprophytic pseudomonads

Article Abstract:

The copper accumulation in copper-resistant strains Pseudomonas species was determined. The bacteria were grown in MGY broth containing concentrations of metal salts, harvested by centrifugation, and freeze-dried then metal content was analyzed by atomic absorption spectrophotometry. Results showed that copper resistance in Psedumonas species is caused by copper sequestration, a mechanism which is common to Pseudomonas species found on plants to which antimicrobial copper was applied. Further tests also showed that P. syriangae strain PS61 containing cop operon accumulated more copper than PS61 without the operon.

Pseudomonas

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Multiple periplasmic catalases in phytopathogenic strains of Pseudomonas syringae

Article Abstract:

Catalase activity was identified in both the periplasm and cytoplasm of Pseudomonas syringae strains. Gel electrophoresis showed multiple bands of activity in all the lysates, indicating possible multiplicity of isozymes. Activity varied with growth phase, with cells in the stationary-growth phase having the highest levels. These cells exhibited induction of activity as well as the ability to survive hydrogen peroxide levels as high as 50 millimoles (mM). Catalase activity for cells in the log phase was inducible up to hydrogen peroxide concentrations of 10 mM, but decreased afterward.

Research, Enzymes

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PsrA, the Pseudomonas sigma regulator, controls regulators of epiphytic fitness, quorum-sensing signals, and plant interactions in Pseudomonas syrinage pv. tomato strain DC3000

Article Abstract:

The study analyzes the effects of PsrA, the Pseudomonas sigma regulator on the Pseudomonas syringe pv. tomato strain DC3000, a pathogen of tomato and Arabidopsis. The study shows that PsrA controls the regulators of epiphytic fitness, quorum-sensing signals, as well as plant interactions in the strains.

Analysis, Genetic aspects, Arabidopsis thaliana, Quorum sensing

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