Acetate kinase from Clostridium acetobutylicum: a highly specific enzyme that is actively transcribed during acidogenesis and solventogenesis

Article Abstract:

A research on the acetate kinase of Clostridium acetobutylicum DSM 1731 was conducted to describe its purification and properties and the transcription of its respective genes during acid and solvent fermentation. C. acetobutylicum DSM 792 and DSM 1731 were primarily subjected to strains, plasmid and growth conditions. Findings of the study showed that under acid and solvent fermentation, C. acetobutylicum sustains produced acids to develop acetone and butanol. Furthermore, the specific activities of cell extracts of the enzymes in acid-producing pathways were found to be reduced.

Research, Enzymes, Enzyme regulation, Enzymatic analysis, Acetate kinase

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Increased pyruvate orthophosphate dikinase activity results in an alternative gluconeogenic pathway in Rhizobium (Sinorhizobium) meliloti

Article Abstract:

Mutations that mediated the ability of Pck-deficient Rhizobium meliloti mutants to utilize succinate and tricarboxylic acid (TCA) intermediates were analyzed to characterize the biosynthesis of phosphoenolpyruvate (PEP). The activity of pyruvate orthophosphate dikinase (PPDK) in Rhizobium (Sinorhizobium) meliloti was enhanced by pckA suppressor mutations. Gluconeogenesis from malate in the pckA suppressor strain was also mediated by the combined activities of malic enzyme and PPDK.

Genetic aspects, Rhizobium, Microbial metabolism, Gluconeogenesis

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Autoinducer 2 activity in Escherichia coli culture supernatants can be actively reduced despite maintenance of an active synthase, LuxS

Article Abstract:

Research shows that the synthase LuxS is dimeric in vivo, which in Escherichia coli directs the generation of autoinducer-2 facilitated by carbohydrate supply. Data indicate that this carbohydrate dependency affects only extracellular autoinducer-2 activity. However, the requiremet for carbohydrae is not seen in in vitro stduies. The primary function of LuxS is metabolic rather than quorum sensing.

United Kingdom, Enzyme synthesis, Cell metabolism, Cell culture

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