Acetogenesis from dichloromethane by a two-component mixed culture comprising a novel bacterium

Article Abstract:

A new bacterium, designated DMC, capable of dehalogenation of dichloromethane and acetogenesis was isolated from a two-component culture that used dichloromethane as its carbon and energy source. The other component, identified as a Desulfovibrio species, may be necessary to provide DMC with a growth factor. DMC was found to be a gram-positive bacterium with a high level of 16S ribosomal DNA sequence similarity with Desulfotomaculum orientis and Desulfitobacterium dehalogenans.

Growth factors

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Metabolism of dichloromethane by the strict anaerobe Dehalobacterium formicoaceticum

Article Abstract:

A study was conducted that examined the metabolism of the chlorinated aliphatic hydrocarbon dichloromethane by the strictly anaerobic gram-positive bacterium Dehalobacterium formicoaceticum. The findings support the hypothesis that the CO dehydrogenase pathway found in cell extracts of D. formicoaceticum is used for the anaerobic metabolism of dichloromethane. It was also found that an activity in cell extracts transforms dichloromethane to methylene tetrahydrofolate.

Physiological aspects

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Chloromethane metabolism by Methylobacterium sp. strain CM4

Article Abstract:

Physiological and genetic studies were conducted to examine the processes involved in the metabolism of chloromethane in Methylobacterium sp. strain CM4, a newly isolated methylotrophic bacterium that utilizes chloromethane. Results indicate that the bacterium strain metabolizes chloromethane first by initial dehalogenation, then by several dehydrogenase-based steps that are unlike those involved in the metabolism of methanol or methylamine in the same bacterium.

Bacteria, Chloromethane, Methyl chloride

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