Extrusion of benzoic acid in Saccharomyces cerevisiae by an energy-dependent mechanism

Article Abstract:

The reaction of Saccharomyces cerevisiae to benzoic acid was analyzed to determine the energy-dependent mechanisms of resistance of yeasts to weak acid preservatives. Results show that the mechanism of extrusion required energy and prior growth in the presence of the inducers. When energy was available, S. cerevisiae released benzoic acid. On the other hand, inhibitor of ATPases such as diethylstilbestrol prevented benzoic acid intrusion.

Saccharomyces, Benzoic acid

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Stress induction of HSP30, the plasma membrane heat stock protein gene of Saccharomyces cerevisiae, appears not to use known-stress regulated transcription factors

Article Abstract:

A study was conducted to show that the heat shock protein, HSP30, is activated by several stresses. Plasmid YIp30 was determined by ligation of the Ndel fragment of pUC19HSP30. Hsp12 mRNA levels were then measured to analyze conditions of nitrogen starvation and their impact on induction. Experimental results indicated that a third system for the activation of genes by heat shock exists in yeast.

Heat shock proteins, Yeast, Yeast (Food product), Cell membranes, Plasma membranes

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