Ammonia-hyperproducing bacteria from New Zealand ruminants

Article Abstract:

The first screening of dairy cows, sheep and deer fed fresh forage for the presence of ammonia-hyperproducing bacteria (HAP) is reported. Five HAP rumen bacteria were isolated and characterized, namely, C2, D1, D4, D5 and S1. 16s ribosomal DNA sequences were tested and analyzed, finding that all isolates were monensin sensitive. D1 and S1 were found to belong to the genus Peptostreptococcus, D4 and D5 to the family Bacteroidaceae, and C2 was found to be very similar to an unidentified species from the genus Eubacterium.

Physiological aspects, Food and nutrition, New Zealand, Ammonia, Ruminants

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Ecology of uncultivated Oscillospira species in the Rumen of cattle, sheep, and reindeer as assessed by microscopy and molecular approaches

Article Abstract:

PCR and PCR-denaturing gradient gel electrophoresis (DGGE) procedures are designed and validated for the detection of Oscillospira spp. The techniques are used to determine the occurrence of this bacterium in different ruminants and during diet shifts in cattle and sheep, and to estimate the genetic diversity of this unique group of bacteria.

Gel electrophoresis, Rumen, Rumen microbiology

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Effect of condensed tannins on bacterial diversity and metabolic activity in the rat gastrointestinal tract

Article Abstract:

The effect of dietary condensed tannins on rat fecal bacterial populations is studied. The results show that condensed tannins of A.angustissima altered fecal bacterial population in the rat gastrointestinal tract causing a shift in predominant bacteria towards other tannin resistant bacteria.

Gastrointestinal system, Tannins

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