An upstream activating sequence containing curved DNA involved in activation of the Clostridium perfringens plc promoter

Article Abstract:

The in vivo transcriptional activity of the plasmid-borne plc gene, which encodes phospholipase C (alpha-toxin) of Clostridium perfringens, was stimulated by a curved-DNA-containing sequence, depending on its proper linear and rotational orientation. The transcriptional activity was also stimulated by the upstream sequence. Moreover, the stimulatory effect of the sequence and the degree of DNA bending were greater at lower temperature. A similar temperature effect was also seen with the chromosomal plc gene.

Genetic aspects

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A method for purification of Clostridium perfringens phospholipase C from recombinant Bacillus subtilis cells

Article Abstract:

Recombinant Bacillus subtilis cells that lack two main proteases can be used to purify phospholipase C (PLC) from Clostridium (C.) perfringens. The method involves SMT medium for culture, three purification steps, and the yield is high. C. perfringens produces many toxins and separating them is difficult, though necessary to study the effect of PLC on the functions of the cells.

Recombinant proteins

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Analysis of genes involved in nitrate reduction in clostridium perfringens

Article Abstract:

Analysis of nitrate reductase operon shows that it is a 9.5 kilobase fragment containing seven open reading frames (ORFs). Nitrate reductase, ferredoxin-like protein, and NADH oxidase are coded by ORF4, 5, and 6 respectively, indicating they are putative genes of nitrate reduction system of Clostridium perfringens.

Japan, Analysis, Usage, Physiological aspects, Genetic regulation, Nitrogen metabolism

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