Purification of pyoverdines of Pseudomonas fluorescens 2-79 by copper-chelate chromatography

Article Abstract:

The absorption and fluorescence spectra of the pyoverdines Pf-A, Pf-B and Pf-C, separated by copper-chelate (EDTA) affinity chromatography from Pseudomonas (P.) fluorescens, depend on pH. The purified pyoverdines are biologically active and exhibit changes in maximal absorbance wavelengths on addition of copper or iron ions. Dissociation of Fe(super +3)-pyoverdine complexes depends on the chelate concentration. The formation of free pyoverdines on incubation of Fe(super +3)-pyoverdine complexes with P. fluorescens indicates the involvement of pyoverdines in iron transport.

Observations, Chelating agents, Pseudomonas fluorescens, Absorption spectra, Plant growth promoting substances, Affinity chromatography

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Fluorescent pseudomonad pyoverdines bind and oxidize ferrous ion

Article Abstract:

Research was conducted to investigate Fe2+-pyoverdine complex formation from Pseudomonas fluorescens, P aeruginosa and P putida using absorption and fluorescence spectroscopic techniques. The objective of the study was to examine the interaction between pyoverdine ligand and ferrous ion. Results indicate that pyoverdines oxidize and bind the ferrous ion. Possible mechanisms of iron removal from iron-pyoverdine complexes by fluorescent pseudomonads were also discussed.

Pseudomonas

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Iron acquisition from transferrin and lactoferrin by Pseudomonas aeruginosa pyoverdin

Article Abstract:

The iron acquisition Pseudomonas aeruginosa PAO1 (ATCC 15692) from transferrin and lactoferrin was investigated. The pathogen was cultured in an iron-deficient medium and was supplemented with different quantities of iron-saturated transferrin and lactoferrin. Results reveal that at physiological pH, P. aeruginosa pyoverdin can require iron from partially iron-saturated transferrin or lactoferrin.

Transferrin, Pseudomonas aeruginosa, Lactoferrins

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