Anaerobic transformation and toxicity of trichlorophenols in a stable enrichment culture

Article Abstract:

The anaerobic degradationof trichlorophenols (TCPs) is mediated by thermophilic spore-forming anaerobes that dechlorinate TCPs in media with complex carbon sources. The reductive dechlorination process is regulated by an inducible enzyme system as shown in studies using chloramphenicol to inhibit enzyme synthesis. Toxicity of chlorophenols to methanogenic bacteria is stoichiometric, that is, the positionof chlorine atoms in the phenol ring corresponds to the degree of chlorophenol toxicity. This toxicity plays a significant part in the transformation of TCPs because it inhibits the dehalogenation reaction to proceed.

Health aspects, Culture media (Biology), Culture media, Chlorophenols

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Isolation and characterization of an anaerobic chlorophenol-transforming bacterium

Article Abstract:

An obligately anaerobic dechlorinating bacterium was purified from a trichlorophenol-dechlorinating consortium selected from municipal sludge. The bacterium was putatively identified as a Clostridium species. Biochemical characterizations showed that the bacterium preferred to dechlorinate the ortho chlorine substituent. Further physiological tests showed that the organism had the ability to grow on pyruvate as sole carbon source, which produced approximately equimolar amounts of acetate.

Analysis, Bioremediation, Clostridium, Identification and classification, Anaerobic bacteria, Organochlorine compounds, Organic chlorine compounds

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Growth physiology of the hyperthermophilic archaeon Thermococcus litoralis: development of a sulfur-free defined medium, characterization of an exopolysaccharide, and evidence of biofilm formation

Article Abstract:

The hyperthermophilic archaeon Thermococcus litoralis grown in defined and complex media produces a sparingly soluble exopolysaccharide with only mannose as the monosaccharidic component. The production of exopolysaccharide by T. litoralis is responsible for the formation of a thin biofilm on polycarbonate filters and glass slides. Growth of the bacteria is unaffected by the addition of elemental sulfur or the presence of H2.

Observations, Polysaccharides

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