Analysis of URS (sub G)-mediated glucose repression of the GAL1 promoter of Saccharomyces cerevisiae

Article Abstract:

Glucose repression by URS (sub G) was investigated through identification of a number of mutants that lacked URS (sub G)-mediated repression. Trans-acting factors of the glucose repression pathway was found to be important for the repression activity of the cis-acting promoter elements. URS (sub G)-mediated glucose repression was not affected by orientation or location within a promoter that indicates the presence of binding sites for glucose-activated repressor proteins. Three new genes needed for the URS (sub G) glucose repression were discovered and were found to encode the repressor proteins.

Genetic regulation, Promoters (Genetics), Glucose, Glucose synthesis

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HTS1 encodes both the cytoplasmic and mitochondrial histidyl-tRNA synthetase of Saccharomyces cerevisiae: mutations alter the specificity of compartmentation

Article Abstract:

A study was conducted to analyze HTS1 encoding of mitochondrial and cytoplasmic histidyl-tRNA synthetases (Hts). Genetic and biochemical experiments indicate a single HTS1 gene is needed for such encoding process. Results show that the gene posseses an amino-terminal presequence in the mitochondrial precursor and a supporting amino-terminal sequence in both precursor and cytoplasmic form. The Hts protein's compartmentation specificity is conditioned by primary sequence and protein concentration.

Transfer RNA, Aminoacyl-tRNA synthetases, Cell compartmentation

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