Application of DNA probes to analysis of bacteriophage distribution patternsin the environment

Article Abstract:

Four DNA probes from phages UT1, M1, F116L and D3 were used to assess the distribution of Pseudomonas aeruginosa-infected bacteriophages in natural samples of lake water, sediment, soil and sewage. The DNA probes could detect homologous sequences from between 10(super 3) to 10(super 4) plaque forming units and from 10(super 6) to 10(super 7) colony forming units of lysogenized bacteria. The results showed that up to 40% of P. aeruginosa in the natural environment contains phage sequences. The sewage samples had the most diverse bacteriophage populations. The results indicate a great role for transduction and lysogeny in populations of bacteria from the natural environment.

Usage, Demographic aspects, Genetic aspects, Bacteriophages, DNA probes, Pseudomonas aeruginosa, Lysogeny

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Enterocin P selectively dissipates the membrane potential of Enterococcus faecium T136

Article Abstract:

Enterocin P reduces the membrane potential and the intracellular ATP pool of Enterobacter faecium T136 but did not affect the pH component of the proton motive force. Enterocin P is a bacteriocin produced by Enterococcus faecium P13 and other E. faecium strains.

Bacterial toxins

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Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates

Article Abstract:

Strains of Enterococcus used as starters and as probiotics in food can acquire virulence determinants from strains isolated from patients via transconjugation. Enterococcus can cause disease in humans, and it is becoming resistant to some antibiotics.

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