Assessment and interpretation of bacterial viability by using the LIVE/DEAD BackLight kit in combination with flow cytometry

Article Abstract:

The application of propidium iodide in combination with a green fluorescent total nucleic acid stain on UVA-irradiated cells of Escherichia coli, Salmonella enterica serovar Typhimurium, Shigella flexneri, and a community of fresh water bacteria resulted in a clear and distinctive flow cytometric staining pattern.

Analysis, Flow cytometry, Bacterial cell walls

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Molecular subtyping and genetic analysis of the enterohemolysin gene (ehxA) from Shiga toxin-producing Escherichia coli and atypical enteropathogenic E. coli

Article Abstract:

The distribution of enterohemolysin subtypes, a plasmid-encoded toxin, among Escherichia coli isolates from various human and animal sources is assessed. No apparent difference that can indicate specific roles for enterohemolysin in the separate hosts is found.

Hemolysis and hemolysins, Hemolysis

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Photoreactivation and dark repair in UV-treated microorganisms: Effect of temperature

Article Abstract:

The study related to the effect of salinity and temperature on the reactivity of Escherichia coli in a marine environment showed the effect of salinity to be greater than that of temperature.

Usage, Marine ecology

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