Automated confocal laser scanning microscopy and semiautomated image processing for analysis of biofilms

Article Abstract:

A computer program for investigating biofilms through image acquisition and analysis techniques is presented. The computer-based approach primary involves the on-line collection of confocal 2D cross-sectional images from a reset 3D domain and then the off-line analysis of the 2D images. The method was tested by applying it to two different pure-culture biofilms. Results indicate that the computer-controlled processing is effective.

Microorganisms, Image processing

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Natural genetic transformation in monoculture Acinetobacter sp. strain BD413 biofilms

Article Abstract:

Research reveals that effective natural genetic transformation occurs in the Acinetobacter sp. strain BD413 biofilms without any barrier. Results show that a 15 minute exposure is adequate for transformation and detectable transformation can be accomplished with as little as 2.4 fentogram DNA. Furthermore, young and actively growing biofilms show highest transfer frequencies with high DNAconcentrations.

United States, Biological Product (except Diagnostic) Manufacturing, Drugs, Deoxyribonucleic Acid, DNA, Bioremediation, Microbial mats, Genetic transformation

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Evaluation of the use of PCR and reverse transcriptase PCR for detection of pathogenic bacteria in biosolids from anaerobic digestors and aerobic composters

Article Abstract:

The authors have investigated pathogenic bacteria. The aim of their research has been to detect these bacteria in organic waste via PCR-based and reverse transcriptase PCR-based methods using Salmonella spp., Yersinia enterocolitica, Listeria monocytogenes and Staphylococcus aureus as model organisms.

In-Vitro Diagnostic Substance Manufacturing, Diagnostic substances, Reverse Transcriptase, Analysis, Environmental aspects, Genetic aspects, Polymerase chain reaction, Microbiology, Bacteria, Pathogenic, Pathogenic bacteria, Organic wastes, Microbial populations, Microbial ecology

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