Biosynthesis of poly(3-hydrozyalkanoic acid) copolymer from CO2 in Pseudomonas acidophila through introduction of the DNA fragment responsible for chemolithoautotrophic growth of Alcaligenes hyrogenphilus

Article Abstract:

A study was conducted on the production of poly(3-hydroxyalkanoic acid) (PHA) from carbon dioxide using two approaches. In the first method, PHA biosynthetic genes accumulating PHA from low-molecular-weight organic compounds were introduced into a hydrogen-oxidizing bacterium. In the second method, the chemolithoautotrophic growth ability of a hydrogen-oxidizing bacterium was introduced into a PHA-producing bacterium. Both approaches proved to be unique and effective in carbon dioxide recycling.

Block copolymers, Organic acids

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Effect of compressed carbon dioxide on microbial cell viability

Article Abstract:

Research was conducted to examine the influence of compressed carbon dioxide on the survival of Escherichia coli, Enterococcus faecalis and Saccharomyces cerevisiae over a range of pressures from 1.5 to 5.5 MPa and exposure times of up to 7 h. The objective was to study the feasibility of using a new pressurizable reactor system to destroy microorganisms. Results revealed the existence of a linear correlation between CO2 pressure and exposure time and microbial inactivation.

Design and construction, Bioreactors

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Alcaligenes eutrophus as a bacterial chromate sensor

Article Abstract:

A bacterial chromate sensor was developed to characterize the determinants encoding inducible resistance to chromate and to cobalt and nickel in Alcaligenes eutrophus CH34. A cloned part of plasmid pMOL28, which contains both determinants, was mutated with Tn5-lacZ to develop metal-sensing bacterial strains. Results showed that A. eutrophus AE104(pEBZ141) could be used as a biosensor for chromate when cultivated in glycerol as an optimal carbon source.

Biosensors, Nickel, Cobalt

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