Characterization of plasmid pOR1 from Ornithobacterium rhinotracheale and construction of a shuttle plasmid

Article Abstract:

The isolation and characterization of the native plasmid pOR1 from O.rhinotracheale and the construction of a shuttle vector that can replicate both in Escherichia coli and Ornithobacterium rhinotracheale are presented. Studies demonstrate that shuttle vector paves the way for genetic modification and characterization of O.rhinotracheale genome and for the development of genetically defined vaccine strains.

Science & research, Research, Genetic aspects, Escherichia coli, Plasmids, Genetic research

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Identification of the cadmium-inducible Hansenula polymorpha SEO1 gene promoter by transcriptome analysis and its application to whole-cell heavy-metal detection systems

Article Abstract:

A set of Hansenula polymorpha (Hp) SEO1 genes was analyzed for selecting genes suitable for constructing cellular cadmium biosensor. Results conclude that an HpSEO1 gene promoter was substantially useful for monitoring heavy metal contamination.

Primary nonferrous metals, not elsewhere classified, Primary Smelting and Refining of Nonferrous Metal (except Copper and Aluminum), Cadmium, Analysis, Usage, Biosensors, Genetic transcription, Transcription (Genetics)

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