Characterization of the variable-number tandem repeats in vrrA from different Bacillus anthracis isolates

Article Abstract:

PCR analysis of 198 Bacillus anthracis isolates indicated a variable region of DNA sequence differing in length among the isolates. Five polymorphisms were characterized by the presence of two to six copies of the 12-bp tandem repeat 5'-CAATATCAACAA-3'. This variable-number tandem repeat region is found within a larger sequence containing one complete open reading frame that encodes a putative 30-kDa protein. Length variation did not alter the reading frame of the encoded protein and only modified the copy number of a 4-amino-acid sequence (QYQQ) from 2 to 6.

Anthrax

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Fluorescent amplified fragment length polymorphism analysis of Norwegian Bacillus cereus and Bacillus thuringiensis soil isolates

Article Abstract:

Researchers used fluorescent amplified fragment length polymorphism to analyze 154 Bacillus cereus and Bacillus thuringiensis isolates taken from five different locations and compare them to reference strains. This method can be automated, allowing scientists to process very large samples.

Soil microbiology

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Differentiation of Clostridium botulinum serotype A strains by multiple-locus variable-number tandem-repeat analysis

Article Abstract:

Variable-number tandem-repeat (VNTR) markers are used for differentiating among the clostridial strains. These markers have provided a rapid, highly discriminatory tool for distinguishing among Clostridium botulinum botulism neurotoxin A1 (BoNT/A1) strains for examining botulism outbreaks.

Science & research, Clostridium, Genetic markers, Botulinum toxin, Botulinum toxins

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