Characterization of thermophilic consortia from two souring oil reservoirs

Article Abstract:

The microbial consortia from produced-water at two different oilfields in Kuparuk, Alaska, and Ninian, North Sea, are capable of sulfate reduction of different substrates over a range of temperatures. Temperature affects the rates of sulfate reduction, substrate oxidation, hydrogen sulphide production and cell morphology. The highest rates of sulfate reduction and substrate oxidation occurs between 50 and 60 degree celsius. The consortium from the Alaska field can produce methane from hydrogen and carbon dioxide at mesophilic and thermophilic temperatures.

Analysis, Physiological aspects, Oil fields, Bacteria, Thermophilic, Thermophiles

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Direct cloning from enrichment cultures, a reliable strategy fo isolation of complete operons and genes from microbial consortia

Article Abstract:

A process is described for constructing cosmid libraries from microbial consortia with large inserts, using a combined approach involving direct cloning and enrichment cultures. The process can be used for studying microbial populations for diverse catabolic and metabolic activities on a molecular level, with potential applications in biotechnology.

Germany, Statistical Data Included, Methods, Usage, Identification and classification, Cultures (Biology), Cloning, Operons, Biotechnology, Microbial genetics

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Genetic and functional analysis of the tbc operons for catabolism of alkyl- and chloroaromatic compounds in Burkholderia sp. strain JS150

Article Abstract:

Burkholderia sp. strain JS150 has several alkyl- and chloroaromatic monooxygenases. The product of the tbc1 gene is homologous to multicomponent cresol and phenol hydroxylases and the product of the tbc2 gene is homologous to toluene and benzene monooxygenases.

Biodegradation, Microbial enzymes

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