Genetic manipulation of Bacillus methanolicus, a gram-positive, thermotolerant methylotroph

Article Abstract:

The first genetic transformation system, shuttle vectors and integrative vectors for the thermotolerant, methylotrophic bacterium Bacillus methanolicus are presented. Two low-copy-number Escherichia coli-B. methanolicus shuttle plasmids, pDQ507 and pDQ508 are shown. pDQ508 contains the replication origin cloned from a 17-kb endogenous B. methanolicus plasmid, pBM1. pDQ507 contains a cloned B. methanolicus DNA fragment, pmr-1, possibly of chromosomal origin, that supports maintenance of pDQ507 as a circular, extrachromosomal DNA molecule.

Genetic aspects, Bacillus (Bacteria), Bacillus

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Role of the Bacillus methanolicus citrate synthase II gene, citY, in regulating the secretion of glutamate in L-lysine-secreting mutants

Article Abstract:

Results indicate that Bacillus methanolicus exhibit several forms of citrae synthase and glutamate secretion can be altered to increase L-lysine secretion by regulaing citrate synthase activity. In the citrate synthase deficient-mutant , the level of of glutamate secreted is reduced 7-fold and the ratio of L-lysine to glutamate secreted is enhance 4-5-fold as compared to the wild type.

United States, Science & research, Norway, Research, Physiological aspects, Microbial metabolism, Microbial enzymes, Secretion, Enzyme regulation

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