Cloning and partial characterization of regulated promoters from Lactococcus lactis Tn917-lacZ integrants with the new promoter probe vector, pAK80

Article Abstract:

The lactococcus lactis bacteria is an industrial microorganism used to make many different cheeses and cultured milk products. Genetic modification of this bacteria might be used to improve the flavor of some industrially important strains. A lactococcus lactis Tn917-LTV1 integrant was produced from the transposon Tn917-LTV1 gene by fusing chromosomal loci with the promoterless lacZ gene. Insertion of one of the promoters in pAK80 indicated a regulated beta-galactosidase expression similar to the regulation found in Tn917-LTV1 integrant.

Industrial microorganisms

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Efficient transformation system for Propionibacterium freudenreichii based on a novel vector

Article Abstract:

Researchers used a 3.6-kb plasmid from Propionibacterium freudenreichii to insert an E.coli-specific replicon and an erythromycin resistance gene into the bacterium. This bacterium is used for industrial purposes, so a means of genetically modifying the bacterium would be useful.

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A food-grade cloning system for industrial strains of Lactococcus lactis

Article Abstract:

Researchers discuss the food-grade cloning vector pFG200, which can be used to express a variety of genes in Lactococcus lactis. The vector has no adverse effect on the bacterial growth rate or the rate of acidification in milk.

Food, Agricultural biotechnology

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