Cloning of the ribosomal protein L41 gene of Phaffia rhodozyma and its use as a drug resistance marker for transformation

Article Abstract:

The L41 protein gene of the fermenting yeast Phaffia rhodozyma was cloned and then used as a cycloheximide resistance marker during the transformation of the yeast. Transformation efficiencies of 800 to 1,200 transformants per microgram of DNA were obtained. Expression of the cycloheximide resistance gene was achieved through postincubation of electroporated cells for 14 to 16 hours. Homologous recombination into the rDNA locus appeared to be the course by which plasmid integration was accomplished in most transformants.

Cloning, Yeast, Yeast (Food product), Ribosomal proteins

---

Electrical energy changes conductivity and determines optimal electrotransformation freqency in Gram-negative bacteria

Article Abstract:

The conditions required for optimal electroporation efficiency in Gram-negative bacteria were determined. Experiments carried out on three species of Gram-negative bacteria showed that resistance of the bacterial suspensions fluctuates by four orders of magnitude during capacitor discharge. Furthermore, independent manipulations of pulse duration and capacitor time constants revealed that electrical energy delivered in each pulse is the major factor determining electrotransformation efficiency.

Gram-negative bacteria, Bioelectrochemistry

---

Natural transformation in river epilithon

Article Abstract:

Free DNA (lysates) or live donor cells naturally transform strains of the Acinetobacter calcoaceticus, incorporated in situ in river epilithon, to prototrophy. The age of the recipient indigenous community affects the transfer frequency in a laboratory setting. Though the transfer frequency is positively related with the temperature, river Taff below 10 degrees celsius fail to show any transformation. However, laboratory microorganisms are unable to reflect the actual environmental conditions.

Water, Aquatic microbiology, Marine bacteria, Microbial genetics

Similar abstracts:

• Abstracts: Power of a chromosomal test to detect genetic variation using genetic markers. A multivariate approach to the problem of QTL localization
• Abstracts: Tbx3 controls the sinoartrial node gene program and imposes pacemaker function on the atria. Activation of the Notch-regulated transcription factor CBF1/RBP-Jk through the 13SE1A oncoprotein
• Abstracts: Inheritance of the morphological differences between maize and teosinte: comparison of results for two F2 populations
• Abstracts: Expression of the maize MnSod (Sod3) gene in MnSOD-deficient yeast rescues the mutant yeast under oxidative stress
• Abstracts: Changes in bacterial composition and enzymatic activity in ileostomy and ileal reservoir during intermittent occlusion: a study using dogs

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.