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Cloning the soil metagenome: a strategy for accessing the genetic and functional diversity of uncultured microorganisms

Article Abstract:

Research has been conducted on microbial diversity in nature. The libraries of genomic DNA isolated from soil have been constructed to investigate the extent of microbial diversity.

Author: Rondon, Michelle R., August, Paul R., Bettermann, Alan D., Brady, Sean F., Grossman, Trudy H., Liles, Mark R., Loiacono, Kara A., Lynch, Berkley A., MacNeil, Ian A., Minor, Charles, Tiong, Choi Lai, Gilman, Michael, Osburne, Marcia S., Clardy, Jon, Handelsman, Jo, Goodman, Robert M.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
Statistical Data Included, Analysis, Microorganisms, Cloning, Microbiological research

User Contributions:

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Chiranjit Maity
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Aug 4, 2008 @ 4:04 am
Metagenomic study, one of my interested field. I want to apply this in case of uncultured flora in faeces.

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A census of rRNA genes and linked genomic sequences within a soil metagenomic library

Article Abstract:

Research has been conducted on microbial genomes from the library of soil metagenomic DNA. The diversity of these genomes has been investigated, and the genomic sequence isolated from uncultured bacteria has been described.

Author: Liles, Mark R., Handelsman, Jo, Goodman, Robert M., Manske, Brian F., Bintrim, Scott B.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
Physiological aspects, Environmental aspects, Genetic aspects, Soils, Bacteria, Microbiology, Ribosomal RNA, Microbial genetics, Microbial ecology

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Long-chain N-acyltyroine synthases from environmental DNA

Article Abstract:

Nine additional long-chain N-acyltyrosine-producing clones were characterized to explore the genetic diversity underlying the production of the apparently common natural products. The heterologous expression of eDNA in easily cultured hosts provided access to many of the previously inaccessible natural products.

Author: Brady, Sean F., Clardy, Jon, Chao, Carol J.
Publisher: American Society for Microbiology
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
Bacterial genetics

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Subjects list: Research, United States, DNA, Genomes
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