Rapid and simple determination of the Escherichia coli phylogenetic group

Article Abstract:

Research describes a triplex polymerase chain reaction based phylogenetic grouping technique that is rapid and show excellent correlation with reference methods. Results point out that the technique can be used to establish links between phylogenetic group and virulence, as a screening tool, and to identify nonpathogenic strains of Escherichia coli.

Methods, Analysis, Testing, Genetic aspects, Polymerase chain reaction

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Commensal Escherichia coli isolates are phylogenetically distributed among geographically distinct human populations

Article Abstract:

Commensal Escherichia coli isolates have been found to be phylogenetically distributed among geographically separated human populations. Geographic/climatic conditions; or host genetic factors in commensal flora; or dietary factors and/or use of antibiotics may be factors. Data suggest that strains of A, B1 and D phylogenetic groups are the predominate ones in gut flora and. It appears the strains must acquire virulence factors to become pathogenic. Commensal phylogenetic group B2 strains are not often seen but seem to be potentially virulent.

Physiological aspects, Intestines, Intestinal microbiology, Escherichia coli infections, Biogeography

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Shigella and enteroinvasiv Escherichia coli strains are derived from distinct ancestral strains of E. coli

Article Abstract:

A differentiation between the subspecies Shigella and the phylogenetic position of Shigella clones in Escherichia coli clones, has been determined through analysis of restriction fragment length polymorphisms of rDNA. Shigella strains were distantly related to group B2 phylogenetic group containing E. coli strains often implicated in extra-intestinal infections in humans. 13 of the enteroinvasive E. coli strains were distributed more widely between phylogenetic groups B1, A and B2.

Shigella

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