Comparative evaluation of modified m-FC and m-TEC media for membrane filter enumeration of Escherichia coli in water

Article Abstract:

Student,s t test of data from membrane filter enumeration of Escherichia (E.) coli fecal coliforms by using m-FC and m-TEC media does not show any significant difference between the two media. Both of the media were tested with 181 water samples from sewage, rivers, lakes, and wells. A scatter plot of the data demonstrates a strong positive linear correlation between the two enumeration methods. Either of these two media can be used to determine E. coli in nontreated water.

Water, Aquatic microbiology, Filtration, Water quality bioassay

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A new membrane filtration medium for simultaneous detection and enumeration of Escherichia coli and total coliforms

Article Abstract:

Microbiological research shows that the new membrane filtration medium m-ColiBlue24 broth is better than mTEC medium at recovering Escherichia coli. The recovery success of m-ColiBlue24 is evaluated using three protocol modifications: no correction, AP120E and ancillary oxidase. Its relative accuracy is assessed using coliforms in 25 water samples from seven US sites.

Membrane filters, Membrane separation

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Development of a quantitative competitive PCR assay for detection and quantification of Escherichia coli O157:H7 cells

Article Abstract:

Quantitative competitive PCR can be used to measure Escherichia coli O157:H7 in food. This bacterium can cause severe food poisoning. Regular PCR cannot assess the number of bacterial cells because it amplifies genes exponentially.

Innovations, Food, Food microbiology, Polymerase chain reaction, Bacteriology

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