Comparison of the recoveries of Escherichia coli and total coliforms from drinking water by the MI agar method and the U.S. Environmental Protection Agency-approved membrane filter method

Article Abstract:

The MI agar method for the detection of Escherichia coli and coliforms in drinking water is better than the conventional method approved by the U.S. Environmental Protection Agency. The MI method uses indoxyl-beta-D-glucuronide and 4-methylumbelliferyl-beta-D-galactopyranoside for the simultaneous determination of E. coli and total coliforms. The recoveries of E. coli and coliforms by this method are higher than that by the conventional method. The tap or wastewater source and overall chlorine levels have no influence on the method's efficiency.

Management

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Evaluation of indoxyl-beta-D-glucuronide as a chromogen in media specific for Escherichia coli

Article Abstract:

Indoxyl-Beta-D-Glucuronide (indoxyl) was determined as a specific chromogen for tracing Escherichia coli by the membrane filter method. The 413 colonies examined from the indoxyl-supplemented media, 93.3 percent gave positive results. Other media gave lower recovery with high verification or equal recovery with poor verification on comparison with the indoxyl medium. Samples from two wastewater treatment plants and two sites along the Ohio River were considered for the confirmation of the presence Escherichia coli contamination in them.

Research, Evaluation, Chromogen

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Occurrence of nontuberculous mycobacteria in environmental samples

Article Abstract:

Nontuberculous mycobacteria (NTM) are a major cause of opportunistic infection in immunocompromised hosts. Because there is no evidence of person-to-person transmission and NTM have been found in drinking water, the environment is considered a likely source of infection. The occurrence of NTM in drinking water, bottled water and ice samples was examined using membrane filtration culture technique followed by polymerase chain reaction amplification and 16S rRNA sequence determination to identify the isolates.

Health aspects, Environmental aspects, Bottled drinking water, Mycobacteria, Mycobacterium, Mycobacterial infections, Mycobacterium infections, Ice, Drinking water, Bottled

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