Conservation of structure and mechanism between eukaryotic topoisomerase I and site-specific recombinases

Article Abstract:

Research was conducted to study the crystallization of the C-terminal catalytic domain Topo(81-314) and the determination of its structure. Topo(81-314) was determined in Escherichia coli BL21 cells under the influence of a T7 promoter. Protein expression was conducted by the infection of an 8 I culture with bacteriophage lambda CE6. Results showed that the C-terminal catalytic domain was characterized by a protease-sensitive region from residues 135-147.

Genetic aspects, DNA topoisomerase I, Vaccinia

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Positional information within the Mu transposase tetramer: catalytic contributions of individual monomers

Article Abstract:

Research indicates that the reactions involved in Mu transposition are mediated by Mu transposase monomers contained in Mu protein tetramers. Reactions between a pair of Mu inactive variants show that the Mu monomers are contained in the transpositional complex serving as a factor for nucleoside splitting and in the transferring of DNA (Deoxiribonucleic acid) strands. MuA binding elements also contain both domain II for R1 and domain III alpha for R2.

Bacteriophages

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The 3' enhancer region determines the B/T specificity and pro-B/pre-B specificity of immunoglobulin V(sub kappa)-J(sub kappa) joining

Article Abstract:

The immunoglobulin kappa gene 3' enhancer (E3') suppresses V(sub kappa)-J(sub kappa) recombination and restricts it to the pre-B developmental stage of B cells. The B/T specificity of V(sub kappa)-J(sub kappa) recombination is controled by the PU.1 binding domain in E3'. A mutation in the PU.1 domain causes recombination in both B and T cells. Deletion of E3' causes rearrangement of the kappa gene in the early pro-B stage of B cells.

Immunoglobulin genes, Immunospecificity

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