Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets

Article Abstract:

An overrepresentation of conserved adenosines flanking the seed complementary sites in mRNAs indicates that primary sequence determinants could supplement base pairing to specify microRNA (miRNA) target recognition. In a four-genome analysis of 3' UTRs, approximately 13,000 regulatory relationships were detected above the estimate of false-positive predictions, thereby implicating as miRNA targets more than 5,300 human genes, which represented 30% of the gene set.

Messenger RNA, Adenosine, Human genome

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Prediction of mammalian microRNA targets

Article Abstract:

This article describes an algorithm-based approach to predict mammalian microRNAs to deduce microRNA:target relationship in animals. Research indicates that pairing to the 5' portion of the microRNA at the 2-8 nucleotide region seems to be the target recognition site by vertebrate microRNAs. Data suggest that the mammalian microRNA targets are enriched for genes mediating transcriptional regulation.

Analysis, Usage, Genetic aspects, Genetic regulation, Genetic transcription, Transcription (Genetics), RNA, Genetic algorithms, Genes

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Passenger-strand cleavage facilitates assembly of siRNA into Ago2-cointaning RNAi enzyme complexes

Article Abstract:

Argonaute (Ago2) receives the double-stranded siRNA from the RNA-induced silencing complexes (RISC) assembly machinery directly is shown. Ago2 cleavages the siRNA passenger strand and liberates the single-stranded guide and for all siRNAs all RISC is assemsebled through the cleavage assisted mechanism.

All Other Basic Organic Chemical Manufacturing, Enzymes, Other Basic Organic Chemical Manufacturing, Enzymes for Food Processing, RNA interference

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