Construction and characterization of a 1,3-propanediol operon

Article Abstract:

A study was conducted to determine the characterization of the genes and enzymes responsible for the conversion of glycerol to 1,3-propanediol (PD) operon. Enhancement of the utility of the 1,3-PD genes was analyzed through their rearrangement into a form that would be adaptable to expression in different hosts under any desired regulation. The study revealed that the 1,3-PD operon was designed so that it can be readily altered for expression in other prokaryotic hosts and is therefore helpful in metabolic engineering of 1,3-PD pathways from glycerol and other substrates.

Genetic aspects, Klebsiella, Glycerol

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Use of an ipb-lux fusion to study regulation of the isopropylbenzene catabolism operon of Pseudomonas putida RE204 and to detect hydrophobic pollutants in the environment

Article Abstract:

Plasmid pOS25 was constructed to investigate the regulation of the isopropylbenzene (ipb) catabolism operon. The plasmid contains the regulatory elements of the ipb operon, namely ipbR and ipbo/p. Inducers of the ipb operon were found to be hydrophobic compounds and were also observed to include monoalkylbenzenes, substituted benzenes and toluenes, alkanes and cycloalkanes, chlorinated solvents and naphthalenes.

Metabolism

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