Construction of a sorbitol-based vector for expression of heterologous proteins in Saccharomyces cerevisiae

Article Abstract:

Promoter and terminator sequences from the yeast Saccharomyces cerevisiase SOR1 gene was used to construct the new inducible yeast expression vector pXS7. The SOR1 gene codes the highly expressed sorbitol-inducible enzyme sorbitol dehydrogenase. The utility of the new vector is demonstrated by placing the coding sequence of a yeast translation elongation factor gene, YEF3, under the control of the promoter and terminator regions of the SOR1 in pXS7 to overexpress the coding sequence. YEF3 protein levels were found to increase when cells were grown with the sugar sorbitol.

Genetic vectors, Bacterial proteins, Sorbitol

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Partial deletion of the Saccharomyces cerevisiae GDH3 gene results in novel starvation phenotypes

Article Abstract:

A large deletion of Saccharomyces cerevisiae GDH3 gene, encoding NADP-linked glutamate dehydrogenase, results in unregulated starvation phenotypes in both haploid and diploid cells. In rich solid media, haploid mutants produce wrinkled colonies comprising of a high degree of pseudohyphae and other aberrant cell types. On poor media, haploids lose their viability. Heterozygous diploids, carrying the deletion at the GDH3 location, sporulate well on rich media and show partial meiotic lethality.

Observations, Nitrogen metabolism

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Filamentous growth of the budding yeast Saccharomyces cerevisiae induced by overexpression of the WHI2 gene

Article Abstract:

Epitope tagging of Whi2 was conducted to determine whether or not the filamentous growth of the budding yeast Saccharomyces cerevisiae is induced by the overexpression of the Whi2 gene. Results reveal that overexpressing Whi2 leads to a concerted reorganization of the cell cycle and promotes filamentous growth wherein cells fail to complete cytokinesis and the budding pattern changes from axial to polar. Furthermore, another site suppressor fsw1 also caused a similar phenotype.

Cell cycle

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