The localization of chitin synthase in membranous vesicles (chitosomes) in Neurospora crassa

Article Abstract:

The chitin synthase of Neurospora (N) crassa is present in chitosomes which are abundant near the hyphal tip. The localization of chitin synthase, identified through polyclonal anti-chitin synthase antibodies, shows a band of approximately 110 kDa. Several bands that represent less abundant chitin synthase are seen in cell-free extracts of N. crassa. No chitin synthase is observed in the plasma membrane. However, young hyphal tips and protoplasts of N. crassa contain membrane-associated chitin synthase.

Observations, Microbial enzymes, Membranes (Biology)

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A Neurospora crassa mutant altered in the regulation of L-amino acid oxidase

Article Abstract:

Gln-1bR8 strain was isolated as a parental train to understand the regulation of L-amino acid oxidase in Neurospora crassa mutants. Results show that gln-1bR8 mutant overcame the inhibitory effect of DL-methionine-SR-sulfoximine (MSO) by increasing the activity of L-amino acid oxidase. Furthermore, the L-amino acid oxidase of the MSO mutant was resistant to glutamine repression but was repressed by ammonium.

Oxidases, Amino acids, Nitrogen metabolism

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Changes in fatty acid composition of Neurospora crassa accompany sexual development and ascospore germination

Article Abstract:

Research was conducted to examine fatty acid composition in Neurospora crassa during various stages of its ascospore germination and sexual development. Results indicated that the sexual tissues of the fungi exhibit different fatty acid composition compared with asexual tissues. Moreover, a correlation exists between the stage of sexual development and large-scale changes in fatty acids.

Fatty acids

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