Three-dimensional localization of wild-type and myosin II mutant cells during morphogenesis of Dictyostelium

Article Abstract:

Individual Dictyostelium amoebae can be localized and tracked at high resolution to study development within the multicellular organism, using a fluorescent dye for labelling. The technique has been used to determine why mutant amoebae without myosin II fail to accomplish morphogenesis. Labeled wild-type cells are randomly distributed in the organism and finish normal development. Mutant cells without cortical integrity are apparently unable to break the adhesion of wild-type cells, indicating that mutants lacking myosin II are unable to complete morphogenesis, perhaps because of their inability to move effectively within the mass of adhered cells.

Research, Cell adhesion, Myosin

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Involvement of cyanide-resistant respiration in cell-type proportioning during Dictyostelium development

Article Abstract:

Migrating slugs were stained with rhodamine 123, which stains actively respiring mitochondria, and treated with benzohydroxamic acid (BHAM) and propyl gallate, which inhibit cyanide (CN)-resistant respiration, to induce production of unique cell masses. Cells strongly expressed prestalk-specific genes in a position-dependent manner, but showed reduced expression of prespore-specific genes. These results suggest that cell differentiation is strongly correlated with CN-resistant respiration during slug development.

Cell differentiation, Respiration

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3D analysis of cell movement during normal and myosin-II-null cell morphogenesis in dictyostelium

Article Abstract:

Examination of the three-dimensional trajectories of both normal axenic and myosin-II-null cells of Dictyostelium discoideum amebae by time-lapse computational optical-sectioning microscopy reveals that three-dimensional ameboid locomotion requires myosin-II. Locomotion of the amebae is regulated by various distinct, potentially competing cell-guide mechanisms.

Cells, Cell migration

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