Isolation and characterization of ovochymase, a chymotrypsin-like protease released during Xenopus laevis egg activation

Article Abstract:

An easy two-step method that utilizes a soybean trypsin inhibitor-agarose affinity column helps isolate ovochymase, a 30-kDa chymotrypsin-like protease, from the perivitelline space of unactivated Xenopus eggs. The 30-kDa- and a 78-kDa protease present in the column eluant are separated by SDS-PAGE or gel filtration HPLC. The N-terminal amino aid sequence of ovochymase resembles those of other serine proteases. Substrate specificity and active site specific inhibition studies confirm that ovochymase is a member of the serine protease I family.

Physiological aspects, Proteases, Chymotrypsin

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Heading in a new direction: implications of the revised fate map for understanding Xenopus laevis development

Article Abstract:

Using a study on Xenopus laevis, the significance of revised amphibian fate map in understanding axial patterning and embryonic development in amphibians is presented.

Embryology, Experimental, Embryological research, Chromosome mapping, Amphibians, Organogenesis

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Development of the primary mouth in Xenopus laevis

Article Abstract:

Study of physical changes in oral cavity during development of primary mouth of Xenopus laevis is presented.

Commercial Fishing, Fishing, Methods, Fishes, Organs, Culture of, Organ culture

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