In situ, real-time catalytic gene expression: extraction and characterization of naphthalene dioxygenase mRNA transcripts from groundwater

Article Abstract:

The expression of genes encoding naphthalene catabolism in microorganisms found in a coal tar waste-contaminated site have been examined. To carry out the study, better methods for extracting in situ-transcribed mRNA from the microorganisms were first derived. Several polymerase chain reaction primers aimed at targeting the conserved regions of the naphthalene dioxygenases have also been developed. The mRNA transcripts retrieved from the site were also reverse transcribed and amplified using hybridization analysis. The sequences were then studied using digoxigenin-labeled RT-PCR products as probes and the corresponding PCR products of diverse isolates as targets.

Oxidases, Gene expression, Messenger RNA

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Use of field-based stable isotope probing to identity adapted populations and track carbon flow through a phenol-degrading soil microbial community

Article Abstract:

The field-study is used to provide insight into three distinct populations of microorganisms involved in in situ metabolism of phenol and this approach measured (super 13)CO2 respired from [(super 13)C]phenol and stable isotope probing (SIP) of soil DNA at an agricultural field site. The data shows that SIP has the potential to document population shifts caused by substrate preexposure and to follow the flow of carbon through terrestrial microbial food chains.

Science & research, Microbial metabolism, Phenols, Phenols (Class of compounds), Decomposition (Chemistry), Chemical properties

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The naphthalene catabolic (nag) genes of Polaromonas napthalenivorans CJ2: Evolutionary implications for two gene clusters and novel regulatory control

Article Abstract:

Polaromonas naphthalenivorans CJ2, a bacterium found to be responsible for the field biodegradation of naphthalene at a coal tar waste-contaminated site was examined. It was found that the napthalene catabolic genes in P. napthalenivorans CJ2 were divided into one large and one small gene cluster, suggesting that mobile genetic elements were likely involved in creating the novel arrangement of catabolic and regulatory genes in P. napthalenivorans.

Naphthalene, Petrochemical Manufacturing, Environmental aspects, Biodegradation, Catabolism

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