Cutting, splicing, reordering, and elimination of DNA sequences in hypotrichous ciliates

Article Abstract:

Oxytricha nova genomic DNA is extensively processed. A large number of transposon-like elements and short internal eliminated sequences (IESs) is excised and degraded. Macronuclear destined sequences remaining after IES excision are spliced to form complete genes. Copies of spliced genes are also later excised during band-by-band polytene chromosome destruction, forming short, linear molecules with 2.2 kbp average length. Nongenic DNA are subsequently destroyed, and telomere sequences added to gene-sized DNA molecules. Macronuclei consist of replicated gene-sized molecules.

DNA, Cytogenetics

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Relationship between the flagellates and the ciliates

Article Abstract:

Flagellates and the ciliates were studied to evaluate their unicellularity and similarity of structural properties. The ciliates were structurally similar with the dinoflagellates among the flagellates wherein the cortical alveoli of ciliates were similar with the thecal vesicles of the dinoflagellates likewise with the tubular cristae of their mitochondria, the parasomal sac of ciliates to the pusule of dinoflagellates and their feeding apparatuses. The use of nucleotide sequencing data from rRNA place the dinoflagelltes before the ciliates in phylogeny.

Evaluation, Flagella (Microbiology), Flagella, Anatomy, Comparative, Comparative anatomy

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Karyotypic variability in ribosomal DNA subchromosome size among Copodid ciliates, a possible tool to differentiate Colpodid species

Article Abstract:

Pulsed-field gel electrophoresis was used to examine the karyotypic variability among colpodid ciliates. A total of five Colpoda inflata strains were employed. The 18S ribosomal gene was observed at various locations in the electrophoretic pattern, and these size differences in the ribosomal DNA subchromosomal molecular appear to be species specific. This can be an important technique for differentiating colpodid ciliates.

Analysis, Usage, Eukaryotic cells, Cells (Biology), Eukaryotes, Ribosomal proteins

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