Cyt1Aa protein of Bacillus thuringiensis is toxic to the cottonwood leaf beetle, Chrysomela scripta, and suppresses high levels of resistance to Cry3Aa

Article Abstract:

The Cyt1Aa protein from Bacillus thuringiensis subsp. israelensis, which was previously known to be toxic only to mosquitoes and related dipterans, was observed to be highly toxic to the cottonwood leaf beetle Chrysomela scripta. Also, Cyt1Aa was found to counter high levels of resistance in C. scripta which have resistance to Cry3Aa. Substantial cross-resistance to Cry1Ba in the Cry3Aa-resistant strain was also observed despite only 38% amino acid similarity between these two toxins.

Bacterial toxins, Beetles

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Activities of Bacillus thuringiensis insecticidal crystal proteins Cyt1Aa and Cyt2Aa against three species of sheep blowfly

Article Abstract:

A study was conducted to analyze the activities of Bacillus thuringiensis insecticidal crystal proteins against three species of sheep blowfly. Results indicated that the susceptibility of insect larvae to B. thuringiensis crystal proteins correlated with their midgut environment. Findings also showed that its specificity correlated with the presence of specific toxin receptors on the insect midgut epithelium.

Flies, Diptera

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Display of biologically functional insecticidal toxin on the surface of gamma phage

Article Abstract:

The construction of a display system in which the Cry1AC toxin fused to the amino terminus of the capsid protein D of bacteriophage lambda is described. The resultant phage was viable and infectious and the toxin displayed interacted successfully with its natural receptor.

Science & research, Proteins, Bacteriophage lambda

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