Cytochalasin D interferes with contractile actin ring and septum formation in Schizosaccharomyces japonicus var. versatilis

Article Abstract:

The development of septation or cytokinesis was examined in the yeast Schizosaccharomyces japonicus after one of the already formed structures was removed from the sequence of events by cytochalasin D (CD), an inhibitor of actin polymerization. The contractile actin rings (AR) of the microorganism disappeared and the ring formation was inhibited in the presence of CD. Although nuclear division was completed, no new ARs were formed in the presence of CD. However, the removal of CD resulted in the formation of new ARs.

Yeast, Yeast (Food product), Cytokinesis

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Disruption of the actin cytoskeleton in budding yeast results in formation of an aberrant cell wall

Article Abstract:

Light and electron microscopical study of a temperature-sensitive mutant of Saccharomyces cerevisiae, DBY 1693, find that the disruption of the actin cytoskeleton in budding yeast results in the formation of an aberrant cell wall. This aberrant cell wall is of loose texture with bulging glucan microfibrils incompletely masked with amorphous matrix. The localization of wall synthesis is in accordance with an even distribution of submembranous actin in the form of patches.

Analysis, Usage, Saccharomyces, Electron microscopy, Plant cell walls, Cell walls

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Analysis of microtubules and F-actin structures in hyphae and conidia development of the opportunistic human pathogenic black yeast Aureobasidium pullulans

Article Abstract:

Research has been conducted on ascomycetous black yeast Aureobasidium pullulans. The authors have investigated the cytoskeleton of this pathogen in the effort to use it as a target of antifungal therapy, and they report the results.

Japan, Czech Republic, Science & research, Physiological aspects, Genetic aspects, Microbiology, Microtubules, Pathogenic microorganisms, Structure

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