Bipolar localization of the replication origin regions of chromosomes in vegetative and sporulating cells of B. subtilis

Article Abstract:

A copy of the E. coli lactose operon (lacO) cassette was inserted near the replication origin of Bacillus subtilis chromosome to determine the mechanisms of Bacillus subtilis chromosome segregation. The chromosomes of Bacillus subtilis are oriented with their origins near opposite poles during the initial phase of sporulation. The origin-distal region of the forespore chromosome was then translocated to the small sporangial compartment after polar septation. Finally, the replication origin separated near either end of the sporangium after the engulfment of the forespore by the mother cell.

Escherichia coli, Bacillus subtilis, Cell cycle, Chromosomes

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Molecular insights into polyubiquitin chain assembly: crystal structure of the Mms2/Ubc13 heterodimer

Article Abstract:

Research has been conducted on the signaling properties of ubiquitin which depend on the polyubiquitin chain topology. The results of the crystal structure analysis of the Mms2/Ubc13 heterodimer demonstrate the active site of the Ubc13 at the intersection of the channels which are the binding sites for the substrate ubiquitins.

United States, Analysis, Gene mutations, Gene mutation, Proteins, Cell research, Cytological research, Ubiquitin

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